Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Aat Bioquest HHBS [Hanks Buffer with 20 mM Hepes] (100 mL)](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502249795.JPG-250.jpg)
Aat Bioquest HHBS [Hanks Buffer with 20 mM Hepes] (100 mL)
Hanks Balanced Salt Solution with 20 mM Hepes buffer HHBS is used for a variety of cell culture applications such as washing cells transporting cells or tissue samples diluting cells for counting and preparing reagents. This catalog is for 100 mL.
![Research Products International Corp TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60050-100.02.JPG-250.jpg)
Research Products International Corp TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 100 Grams
TRIS Hydrochloride, Tris-HCl, is a buffering agent commonly utilized to maintain a stable pH in experimental procedures.
It is primarily used as a buffering agent for applications that require a slightly alkaline to neutral pH range. Also, It is effective in the pH range of 7.2 to 9.0, making it suitable for a variety of biochemical and molecular biology techniques.
TRIS HCl is commonly used in DNA and RNA extraction, protein purification, enzyme assays, and electrophoresis. It helps maintain the desired pH conditions for enzymatic reactions, stability of nucleic acids, and separation of proteins during electrophoresis.
One of the advantages of TRIS HCl is its ability to resist changes in pH caused by the addition of acids or bases, making it a reliable buffering agent. It also exhibits low ionic strength, which minimizes interference with biological systems and enzymatic activities.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This DMEM-Low glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline
PHOSPHATE Phosphate buffered saline
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
DULBECCOS Dulbeccos Modified Eagles1L
Rockland Immunochemicals 1.0 M Tris HCl pH 8.0 - MB-005
Tris solution is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane. Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.
Rockland Immunochemicals 1X TE pH 8.0 (10 mM Tris HCl, 1.0 mM EDTA) DEPC TREATED - MB-040
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA or RNA. Tris-EDTA buffer is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.
Rockland Immunochemicals 10X SDS-PAGE Running Gel Buffer (0.25 M Tris, 1.92 M Glycine, 1.0% SDS pH 8.3) - MB-017
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearize proteins and to impart a negative charge to linearized proteins. 10X SDS-PAGE Running Buffer is suitable for laboratory involved in protein biochemistry. Visit our newly expanded web site at www.rockland-inc.com for methods using this and other buffers.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
DULBECCOS Dulbeccos Modified Eagles1L
Sigma Aldrich Fine Chemicals Biosciences Tris-Glycine Buffer 10x Co1L
TRIS Tris-Glycine Buffer 10x Co1L
Sigma Aldrich Fine Chemicals Biosciences Phosphate Buffered Saline1L
Phosphate Buffered Saline (PBS) is routinely used as a wash buffer in Western blot and immunoprecipitation (IP) procedures as well as during sample preparation of cell lysates for use in general molecular biology applications.
ILEX LIFE SCIENCES LLC PAN-Biotech HEPES Buffer 1M (100 ml)
HEPES Buffer 1M. QUANTITY: 100 ml. MANUFACTURED BY: Pan-Biotech (Germany). STORAGE: 2 to 8 degrees Celsius. DESCRIPTION: HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent; one of the twenty Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by aerobic respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. COMPOSITION: HEPES 238.31 g/L.
![Alkali Scientific PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659877.jpg-250.jpg)
Alkali Scientific PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt], 1 Kg
PIPES Sodium Salt (Piperazine N,N' bis(2-ethanesulfonic acid) sodium salt) is a laboratory-grade biochemical reagent supplied in a 1 kg volume. PIPES is a widely used buffer in biochemical and molecular biology applications. It maintains a stable pH in a range of 6.1 to 7.5, making it ideal for use in studies requiring stable buffering at physiological pH. It is commonly used in protein electrophoresis, enzyme assays, and RNA research. The sodium salt form of PIPES provides excellent solubility and a low ionic strength, minimizing interference in sensitive experiments. This reagent is also used in cell culture media and biological research where precise pH control is essential for reliable results.
Hampton Research HR2-577/1.0 M HEPES sodium - 100 ml
HR2-577/1.0 M HEPES sodium - 100 ml. Please note items from this supplier are non-returnable.